No.169, Science (Kexue) Avenue,National HI-TECH Industry Development Zone, Zhengzhou, China [email protected] 0086-371-86162511

LIMING PRODUCTS

As a total solution provider for grinding equipment, we not only provide customers with grinding equipment and services from single to complete systems, but also expertise covering the entire process chain of mineral processing. We specialize in customer needs in different fields. The product processing range covers 1-3mm coarse powder, 20-400 mesh fine powder, 400-1250 mesh ultrafine powder and 1250-3250 mesh micropowder.
MORE

annexin v magnetic separation

Annexin V magnetic-activated cell sorting versus swim-up

07/06/2014· Magnetic activated cell sorting. Briefly, the washed spermatozoa were incubated with Annexin V-conjugated microbeads (Miltenyi Biotec, Auburn, CA, USA) at room temperature for 15 min. About 10 μl of microbeads were used for each one million separated cells. The spermatozoa-microbeads suspension was loaded on a separation column containing a

Cited by: 18

Evaluation of sperm recovery following annexin V magnetic

evaluate the sperm cell recovery rate after MACS separation in order to assess the future potential of this method for the preparation of compromised samples. Short communication Evaluation of sperm recovery following annexin V magnetic-activated cell sorting separation

MACS (magnetic activated cell sorting) before or after

31/10/2017· “The effects of magnetic separation on cryopreserved bovine spermatozoa motility, viability and cryo-capacitation status.” Zygote 22(3): 378-386. 12. Glander, H. J. and J. Schaller (1999). “Binding of annexin V to plasma membranes of human spermatozoa: a rapid assay for detection of membrane changes after cryostorage.”

Author: T.S. Bertelli, M. Da Broi, W.P. Martins, R. Ferriani, P.A. Navarro

Utility of Magnetic Cell Separation as a Molecular Sperm

We evaluated the sensitivity and specificity of annexin V-MACS beads for the separation of apoptotic spermatozoa in order to advocate their use in sperm preparation. Sperm samples from 30 men (15 infertile patients and 15 fertile donors) were fractionated by equilibrating with magnetic beads carrying annexin V antibodies. To show if this

位置: 8600 Rockville Pike, Bethesda, MD

FITC,Annexin,V,Apoptosis,Detection,Kit,RUO 556547 | BD

Annexin V is a 35-36 kDa Ca2+ dependent phospholipid-binding protein that has a high affinity for PS, and binds to cells with exposed PS. Annexin V may be conjugated to fluorochromes including FITC. This format retains its high affinity for PS and thus serves as a sensitive probe for flow cytometric analysis of cells that are undergoing

Annexin V Staining Protocol | BD Biosciences-US

Annexin V Blocking. An additional control that may be performed includes preincubation of cell samples with recombinant unconjugated Annexin V, which is included as part of the Annexin V-FITC Apoptosis Detection Kit II (Cat. No 556570). This serves to block Annexin V-FITC binding sites and thus demonstrates the specificity of Annexin V-FITC

MACS® ART Annexin V System Apoptotic sperm cell

Annexin V recognizes an antigen (externalized phosphatidylserine, EPS) in the plasma membrane of apoptotic cells. Apoptotic cell depletion begins with the magnetic labeling of apoptotic cells by the MACS® ART Annexin V Reagent. The labeled cells are then passed through a separation column located in a fixed magnetic field. Unwanted cells are selectively retained in the column.

Annexin V BioLegend

FITC Annexin V Apoptosis Detection Kit with 7-AAD FC RUO . 640922 . 100 tests $215. FITC Annexin V Apoptosis Detection Kit with PI FC RUO . 640914 . 100 tests $215. Pacific Blue™ Annexin V Apoptosis Detection Kit with 7-AAD

Annexin V MicroBead Kit Various cell types MicroBeads

Murine thymocytes were treated with CD95 (anti-Fas) monoclonal antibody for 3 hours at 37 °C. PS-exposing cells were enriched by labeling with Annexin V MicroBeads followed by separation on an MS Column in the magnetic field of a MiniMACS™ Separator. Cell fractions were stained with Annexin V

MACS® ART Annexin V System Apoptotic sperm cell

Annexin V recognizes an antigen (externalized phosphatidylserine, EPS) in the plasma membrane of apoptotic cells. Apoptotic cell depletion begins with the magnetic labeling of apoptotic cells by the MACS® ART Annexin V Reagent. The labeled cells are then passed through a separation column located in a fixed magnetic field. Unwanted cells are selectively retained in the column.

First Pregnancy, Somatic and Psychological Status of a 4

The mixture was loaded on top of the separation column, which was placed in the magnetic field. The column was rinsed with buffer. All the unlabeled (annexin V–negative) nonapoptotic spermatozoa passed through the column. The annexin V–positive (apoptotic) fraction was retained in the column. The column was removed from the magnetic field

位置: 8600 Rockville Pike, Bethesda, MD

Selected references MACS® ART Annexin V System

Annexin V TESA Sperm Separation. AJP Rep. 5(2): e105–e108. Herrero, MB. et al. (2013) Case report: the use of annexin V coupled with magnetic activated cell sorting in cryopreserved spermatozoa from a male cancer survivor: healthy twin newborns after two previous ICSI failures. J

FITC,Annexin,V,RUO 556419 | BD Biosciences-US

Annexin V is a 35-36 kDa Ca2+ dependent phospholipid-binding protein that has a high affinity for PS, and binds to cells with exposed PS. Annexin V may be conjugated to fluorochromes including FITC. This format retains its high affinity for PS and thus serves as a sensitive probe for flow cytometric analysis of cells that are undergoing

Annexin V magnetic-activated cell sorting versus swim-up

Therefore, cells with externalized PS (Annexin V-positive) will bind to these micro-beads when placed into a column containing iron balls and passed through a strong magnetic field; those cells remain in the separation column and retained in the magnetic field [17, 21]. Non-apoptotic cells (Annexin V- negative) with intact membrane remain unlabelled and pass freely through the column.

EasySep™ Dead Cell Removal (Annexin V) Kit

The EasySep™ Dead Cell Removal (Annexin V) kit is designed to deplete apoptotic (Annexin V+) cells from cell culture or tissue preparations by immunomagnetic negative selection. Unwanted cells are labeled with Annexin V, antibodies and magnetic particles, and separated without columns using an EasySep™ magnet. Labeled cells are separated

Evaluation of sperm recovery following annexin V

Short communication Evaluation of sperm recovery following annexin V magnetic-activated cell sorting separation Short communication Sperm recovery following annexin V cell sorting TM Said et al. Materials and methods Sample preparation Following approval of the institution review board (IRB), semen samples were collected from 19 healthy donors with semen parameters exceeding World

Annexin V MicroBead Kit Various cell types MicroBeads

Murine thymocytes were treated with CD95 (anti-Fas) monoclonal antibody for 3 hours at 37 °C. PS-exposing cells were enriched by labeling with Annexin V MicroBeads followed by separation on an MS Column in the magnetic field of a MiniMACS™ Separator. Cell fractions were stained with Annexin V

Apoptotic Cell Isolation Kit | K258 | BioVision, Inc.

The Apoptotic Cell Isolation Kit provides a simple and efficient means for isolation of apoptotic cells or removal of dead cells from cell culture or tissue preparations using annexin V/magnetic beads (MagBeads). Annexin V is a Ca²ᶧ-dependent phospholipid binding protein with high affinity for phosphatidylserine (PS), which is redistributed

ANDROLOGY LAB CORNER: Utility of Magnetic Cell

Wiley Online Library will be unavailable on Saturday 30th July 2016 from 08:00-11:00 BST / 03:00-06:00 EST / 15:00-18:00 SGT for essential maintenance.Apologies for the inconvenience.

The effects of magnetic separation on cryopreserved bovine

suspended in 80 l of annexin V buffer per 107 cells. Next, 20 l of MACS annexin V microbeads were added per 107 total cells. The mixture was incubated for 15 min in the dark at 10 C. The sperm were then washed with 1–2 ml buffer per 107 cells and resuspend in 500 8l buffer per 10 total cells. The cells were added to the separation column

(PDF) Sperm selection for ICSI using annexin V

Annexin V MACS separation. Left : Generation of EPS-negative sperm by depleting EPS-positive sperm with Annexin V microbeads in the MS column in the magnetic fi eld. Right : After collection of

Annexin V MicroBead Kit Various cell types MicroBeads

Murine thymocytes were treated with CD95 (anti-Fas) monoclonal antibody for 3 hours at 37 °C. PS-exposing cells were enriched by labeling with Annexin V MicroBeads followed by separation on an MS Column in the magnetic field of a MiniMACS™ Separator. Cell fractions were stained with Annexin V

Case report: the use of annexin V coupled with magnetic

Annexin V has specific affinity to phosphatidylserine, therefore it can bind to the apoptotic cells. When annexin V is coupled to superparamagnetic beads and incubated with spermatozoa, the fraction that is annexin V- positive can be separated and removed from the rest of the sperm sample [14,31]. The fraction enriched with non-apoptotic

位置: 8600 Rockville Pike, Bethesda, MD

Demonstration of Performance Timing of Magnetic Separation

important, Viahance does not contain foreign proteins (such as Annexin-V) and is provided autoclaved. Demonstration of Performance –Timing of Magnetic Separation The timing of magnetic separation is an important parameter to consider when using a simple button magnet for dead cell removal. When magnetic separation is too short, all the dead cells are not removed. When magnetic separation is

Advantage of combining magnetic cell separation with

The role of deregulated apoptosis has been well characterized Advantage of combining magnetic cell separation with sperm preparation techniques Professor Glander completed his studies in biochemistry in 1972 and in medicine in 1974 at the University of Leipzig, Germany. Since 1993, he has been a full Professor in Andrology and the Head of the Department of Andrology at the University of

Isolation of human small bowel intraepithelial lymphocytes

26/04/2004· Figure 3a illustrates the relatively low proportions of CD103 −, CD19 + and CD4 + cells in a representative sample prior to magnetic separation. After Annexin-bead separation , we observed a

Sperm selection using magnetic activated SpringerLink

Nadalini M, Tarozzi N, Di Santo M, Borini A, 27th Annual Meeting of the European Society on Human Reproduction and Embryology (ESHRE), Annexin V magnetic-activated cell sorting versus swim-up for the selection of human sperm in ART: is the new approach better then the traditional one? Hum Reprod. 2011;26 suppl 1:i130. Google Scholar

Annexin,V,Binding,Buffer,,10X,concentrate,RUO

The Annexin V Binding Buffer, 10X concentrate has been formulated for use in flow cytometric assays utilizing Annexin V. The Annexin V Binding Buffer is a 10X concentrate composed of a 0.2 µm sterile filtered 0.1M Hepes (pH 7.4), 1.4M NaCl, and 25 mM CaCl2 solution. Prior to staining cells, an appropriate quantity of a 1X working solution

Activation pattern of caspases in human spermatozoa

Objective. We investigated the activation of caspases 8, 9, 1, and 3 in human ejaculated spermatozoa to study main pathways of apoptosis. Potential functional impact of this phenomenon and possible activation mechanisms were examined by [1] subjecting cells to freezing and thawing, and [2] testing the dependence of caspase activity on membrane integrity.

annexin v magnetic separation

Our Target

————

"Customer-oriented", provides a full range of quality services for our customers;

Our Target

————

"Focus on quality, full-service customers!

Our Target

————

"Service core" most valuable customers tailored products;

Copyright © 2012-2020 by China Liming Heavy Industry Science and Technology Co. LTD All rights reserved